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chicken anti v5  (Novus Biologicals)


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    Structured Review

    Novus Biologicals chicken anti v5
    Chicken Anti V5, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 141 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chicken+anti-v5/DYKDDDDK+Epitope+Tag+Antibody+(L5)/pmc12462514-281-23-31
    Average 96 stars, based on 141 article reviews
    chicken anti v5 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Immunohistochemistry:

    Article Title: Investigation of the molecular-genetic basis for courtship differences between Drosophila melanogaster , Drosophila simulans and their hybrids
    Article Snippet: .. The following primary antibodies were used for immunohistochemistry: rat anti-Fru M (1:200; ( )), mouse anti-nc82 (1∶20; Developmental Studies Hybridoma Bank), mouse anti-FLAG (1:500; Sigma, F1804; for smGDP), chicken anti-myc (1:1000; Invitrogen, A21281; for smGDP), rabbit anti-HA-tag (1:300; Cell Signaling, C29F4; for MCFO and trans-Tango), mouse anti-bruchpilot (1:20, DSHB, nc82), chicken anti-V5 tag 550 (1:500; Novus Biologicals, NB600-379R; for MCFO), mouse anti-GFP (1:100; DSHB GFD-4C9-b; for trans -Tango). .. The secondary antibodies used were Alexa Fluor goat anti-rat 488 (1:1000; for Fru M ), goat anti-mouse 633 (1:500; for FLAG), goat anti-chicken 546 (1:1000; Invitrogen, A11040; for myc), rabbit α-GFP Alexa Fluor 488 (1:600; Invitrogen, A21311), goat anti-rabbit 488 (, 1:500; Invitrogen, 32731; for MCFO), goat anti-mouse 633 (1:500; Invitrogen, A21071, for MCFO), and goat anti-mouse 488 (1:500; Invitrogen, A110001; trans -Tango).

    Incubation:

    Article Title: Viral-mediated knockdown of Atxn2 attenuates TDP-43 pathology and muscle dysfunction in the PFN1 C71G ALS mouse model.
    Article Snippet: The cartridge was run on the Ella Automated Immunoassay system (ProteinSimple, 600–100) using the Simple Plex Runner software. .. Primary antibodies, either rabbit anti-ChAT (Abcam, 178,850), rabbit anti-TDP-43 (Proteintech, 10,782–2-AP), rabbit antipTDP-43 (Cosmo Bio, CAC-TIP-PTD-P07), or chicken anti-V5 (Novus Bio, NB600-379) were incubated for 1-h at RT. .. Slides were incubated at 36 °C with anti-Rabbit NP (Roche, 07425317001) and Anti-NP AP (Roche, 07425325001) probes followed by Discovery Red kit (Roche, 07425333001) were used to visualize ChAT positive areas.

    Article Title: Viral-mediated knockdown of Atxn2 attenuates TDP-43 pathology and muscle dysfunction in the PFN1 C71G ALS mouse model
    Article Snippet: Immunohistochemistry was performed using the fully automated Ventana Discovery Ultra. .. Primary antibodies, either rabbit anti-ChAT (Abcam, 178,850), rabbit anti-TDP-43 (Proteintech, 10,782–2-AP), rabbit anti-pTDP-43 (Cosmo Bio, CAC-TIP-PTD-P07), or chicken anti-V5 (Novus Bio, NB600-379) were incubated for 1-h at RT. .. Slides were incubated at 36 °C with anti-Rabbit NP (Roche, 07425317001) and Anti-NP AP (Roche, 07425325001) probes followed by Discovery Red kit (Roche, 07425333001) were used to visualize ChAT positive areas.

    Recombinant:

    Article Title: Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Brp Developmental Studies Hybridoma Bank (DSHB) Cat# nc82; RRID: AB_2392664 Chicken anti-GFP Thermo Fisher Scientific Cat# A10262; RRID: AB_2534023 FITC-conjugated anti-GFP Rockland Cat# 600-102-215; RRID: AB_218187 Cy3-conjugated goat anti-Hrp Jackson ImmunoResearch Cat# 123-165-021; RRID: AB_2338959 Alexa Fluor 647 goat anti-Hrp Jackson ImmunoResearch Cat# 123-605-021; RRID: AB_2338967 Alexa Fluor 405 goat anti-mouse Thermo Fisher Scientific Cat# A31553; RRID: AB_221604 Alexa Fluor 647 goat anti-mouse Thermo Fisher Scientific Cat# A32728; RRID: AB_2633277 Alexa Fluor Plus 555 goat anti-mouse Thermo Fisher Scientific Cat# A32727; RRID: AB_2633276 Alexa Fluor Plus 488 goat anti-chicken Thermo Fisher Scientific Cat# A11039; RRID: AB_142924 Rabbit anti-V5 NovusBiologicals Cat# NBP2-52653; RRID: AB_3329853 FITC goat anti-HRP Jackson ImmunoResearch Cat# 123-095-021; RRID: AB_2314647 Mouse anti-V5 NovusBiologicals Cat# NBP2-52703; RRID: AB_3331229 AlexaFluorPlus 555 F(ab′)2-goat anti-rabbit IgG (H+L) cross-adsorbed Thermo Fisher Scientific Cat# A48283; RRID: AB_2896347 AlexaFluorPlus 488 donkey anti-mouse IgG (H+L) highly cross-adsorbed Thermo Fisher Scientific Cat# A32766; RRID: AB_2762823 Chemicals, Peptides, and Recombinant Proteins LY354740 Tocris Biosciences 3246 Experimental models: Organisms/strains UAS-DmGluRA-RNAi (pVALIUM20) Bloomington Drosophila Stock Center (BDSC) RRID: BDSC_34872 UAS-attP2 BDSC RRID: BDSC_36303 OK6-Gal4 BDSC RRID: BDSC_64199 w118;+;+/SynapGCaMP6f Newman et al.9 RRID :BDSC_67739 w118;B1/CyOGFP (3xP3-EGFP);+/SynapGCaMP6f Newman et al.9 RRID: BDSC_67740 w;+; nSyb-GAL4.UAS-CD4-tdTomato/+; DmGluRA-V5X5/+ This study N/A w;+; nSyb-GAL4.UAS-CD4-tdTomato/UAS-DmGluRA-RNAi; DmGluRA-V5X5/+ This study N/A w;+; +; DmGluRA-V5X5/DmGluRA-V5X5 This study N/A w; OK6/+; attP2/+; DmGluRA-V5X5/+ This study N/A OK6/+; UAS-DmGluR-RNAi; DmGluRA-V5X5/+ This study N/A Software and Algorithms QuaSOR custom code https://github.com/newmanza/ Newman_QuaSOR_2021 https://doi.org/10.5281/zenodo.5711302 Code written for Aghi et al. 2025 https://github.com/kaghi/ Aghi-et-al-2025_Curr_Bio Zenodo DOI URL: https://doi.org/10.5281/zenodo.15227043 ImageJ National Institutes of Health https://imagej.net/software/fiji/ MATLAB Mathworks https://www.mathworks.com/ products/matlab.html R R Foundation https://www.r-project.org/ Imaris Oxford Instruments https://imaris.oxinst.com/ ll OPEN ACCESS Current Biology 35, 1–12.e1–e6, June 23, 2025 e1 Article ..

    Software:

    Article Title: Balanced synapse-to-synapse short-term plasticity ensures constant transmitter release.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti-Brp Developmental Studies Hybridoma Bank (DSHB) Cat# nc82; RRID: AB_2392664 Chicken anti-GFP Thermo Fisher Scientific Cat# A10262; RRID: AB_2534023 FITC-conjugated anti-GFP Rockland Cat# 600-102-215; RRID: AB_218187 Cy3-conjugated goat anti-Hrp Jackson ImmunoResearch Cat# 123-165-021; RRID: AB_2338959 Alexa Fluor 647 goat anti-Hrp Jackson ImmunoResearch Cat# 123-605-021; RRID: AB_2338967 Alexa Fluor 405 goat anti-mouse Thermo Fisher Scientific Cat# A31553; RRID: AB_221604 Alexa Fluor 647 goat anti-mouse Thermo Fisher Scientific Cat# A32728; RRID: AB_2633277 Alexa Fluor Plus 555 goat anti-mouse Thermo Fisher Scientific Cat# A32727; RRID: AB_2633276 Alexa Fluor Plus 488 goat anti-chicken Thermo Fisher Scientific Cat# A11039; RRID: AB_142924 Rabbit anti-V5 NovusBiologicals Cat# NBP2-52653; RRID: AB_3329853 FITC goat anti-HRP Jackson ImmunoResearch Cat# 123-095-021; RRID: AB_2314647 Mouse anti-V5 NovusBiologicals Cat# NBP2-52703; RRID: AB_3331229 AlexaFluorPlus 555 F(ab′)2-goat anti-rabbit IgG (H+L) cross-adsorbed Thermo Fisher Scientific Cat# A48283; RRID: AB_2896347 AlexaFluorPlus 488 donkey anti-mouse IgG (H+L) highly cross-adsorbed Thermo Fisher Scientific Cat# A32766; RRID: AB_2762823 Chemicals, Peptides, and Recombinant Proteins LY354740 Tocris Biosciences 3246 Experimental models: Organisms/strains UAS-DmGluRA-RNAi (pVALIUM20) Bloomington Drosophila Stock Center (BDSC) RRID: BDSC_34872 UAS-attP2 BDSC RRID: BDSC_36303 OK6-Gal4 BDSC RRID: BDSC_64199 w118;+;+/SynapGCaMP6f Newman et al.9 RRID :BDSC_67739 w118;B1/CyOGFP (3xP3-EGFP);+/SynapGCaMP6f Newman et al.9 RRID: BDSC_67740 w;+; nSyb-GAL4.UAS-CD4-tdTomato/+; DmGluRA-V5X5/+ This study N/A w;+; nSyb-GAL4.UAS-CD4-tdTomato/UAS-DmGluRA-RNAi; DmGluRA-V5X5/+ This study N/A w;+; +; DmGluRA-V5X5/DmGluRA-V5X5 This study N/A w; OK6/+; attP2/+; DmGluRA-V5X5/+ This study N/A OK6/+; UAS-DmGluR-RNAi; DmGluRA-V5X5/+ This study N/A Software and Algorithms QuaSOR custom code https://github.com/newmanza/ Newman_QuaSOR_2021 https://doi.org/10.5281/zenodo.5711302 Code written for Aghi et al. 2025 https://github.com/kaghi/ Aghi-et-al-2025_Curr_Bio Zenodo DOI URL: https://doi.org/10.5281/zenodo.15227043 ImageJ National Institutes of Health https://imagej.net/software/fiji/ MATLAB Mathworks https://www.mathworks.com/ products/matlab.html R R Foundation https://www.r-project.org/ Imaris Oxford Instruments https://imaris.oxinst.com/ ll OPEN ACCESS Current Biology 35, 1–12.e1–e6, June 23, 2025 e1 Article ..

    Imaging:

    Article Title: Synapse-specific catecholaminergic modulation of neuronal glutamate release.
    Article Snippet: .. For OAMB imaging, rat anti- V5 epitope (Novus NBP2- 81037) was used at 1:500. .. Alexa Fluor 488 goat anti- chicken (Thermo Fisher A11039), Alexa Fluor 488 donkey anti- rat (Invitrogen A48272), Alexa Fluor 555 goat anti- rabbit (Thermo Fisher A32727), and Alexa Fluor 647 goat anti- mouse (Thermo Fisher A21235) secondary antibodies were all used at 1:1,000.

    other:

    Article Title: Four neurons pattern brain-wide developmental activity through neuropeptide signaling
    Article Snippet: Brains were finally transferred to Everbrite mounting medium (Biotium 23001) and mounted on to slides for imaging.



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